6A6L

Crystal structure of the cold shock domain of YB-1 in complex with m5C RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97930
Spacegroup name	P 62
Unit cell lengths	66.380, 66.380, 34.702
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.709 - 1.780
R-factor	0.1826
Rwork	0.180
R-free	0.20680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pf5
RMSD bond length	0.006
RMSD bond angle	0.934
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.840
High resolution limit [Å]	1.780	1.780
Rmerge	0.070	0.191
Number of reflections	8518	856
<I/σ(I)>	67.973
Completeness [%]	100.0
Redundancy	17.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2.4M Ammonium sulfate