6A6H
Crystal Structure of Swine Major Histocompatibility Complex Class I SLA-2*040202 For 2.3 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-02-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 101.802, 101.802, 73.455 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 88.160 - 2.310 |
R-factor | 0.2029 |
Rwork | 0.199 |
R-free | 0.27605 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h94 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.033 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.160 | 2.500 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.015 | 0.099 |
Number of reflections | 19686 | 19686 |
<I/σ(I)> | 22.96 | |
Completeness [%] | 89.9 | |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium acetate pH4.6 25% polyethylene glycol 4,000 |