6A6G

Crystal structure of thermostable FiSufS-SufU complex from thermophilic Fervidobacterium Islandicum AW-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979133
Spacegroup name	P 21 21 21
Unit cell lengths	75.265, 76.969, 192.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.616 - 2.490
R-factor	0.14962
Rwork	0.146
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6A6E and 6A6F
RMSD bond length	0.013
RMSD bond angle	1.511
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.129	0.435
Number of reflections	39603
<I/σ(I)>	7.46
Completeness [%]	99.0
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M lithium nitrate, PEG 3350, 0.1M HEPES, pH 7.0