6A67

Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody FLD21.140

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 42 2 2
Unit cell lengths	168.106, 168.106, 147.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.913 - 2.330
R-factor	0.1985
Rwork	0.197
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dut
RMSD bond length	0.009
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.330	2.330
Rmerge	0.091	0.995
Number of reflections	89876	4350
<I/σ(I)>	22.3
Completeness [%]	99.5
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	2.0M (NH4)2SO4. 0.1M Bis-Tris pH 5.5