6A5Q
Structure of 14-3-3 beta in complex with TFEB 14-3-3 binding motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-12 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 0.9789 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 85.170, 85.170, 209.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.880 - 2.000 |
R-factor | 0.1871 |
Rwork | 0.186 |
R-free | 0.22950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bq0 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.805 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.106 | 0.686 |
Number of reflections | 60151 | 5860 |
<I/σ(I)> | 24.9 | 4.7 |
Completeness [%] | 99.5 | 98.8 |
Redundancy | 11.6 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4 | 289 | 0.1 M sodium malonate, and 12% (w/v) PEG 3350 |