6A5E
Crystal structure of plant peptide RALF23 in complex with FER and LLG2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.312, 62.360, 97.478 |
| Unit cell angles | 105.72, 91.30, 108.48 |
Refinement procedure
| Resolution | 42.607 - 2.766 |
| R-factor | 0.2351 |
| Rwork | 0.233 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.298 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.830 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Number of reflections | 32854 | 3150 |
| <I/σ(I)> | 20.89 | |
| Completeness [%] | 97.2 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M sodium malonate, 25% w/v Polyethylene glycol 3350 |
