6A5B
Crystal structure of plant Receptor-like Kinase FER
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-01-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.545, 63.509, 107.776 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.595 - 2.205 |
R-factor | 0.1937 |
Rwork | 0.192 |
R-free | 0.23420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a5c |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 21471 | 2099 |
<I/σ(I)> | 20.4 | |
Completeness [%] | 98.2 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Bis-Tris pH 6.5, 25%(w/v) Polyethylene glycol 3350 |