6A5A
Crystal structure of plant Receptor-like Kinase ANX1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.337, 77.546, 99.288 |
| Unit cell angles | 90.00, 100.52, 90.00 |
Refinement procedure
| Resolution | 41.860 - 2.942 |
| R-factor | 0.1996 |
| Rwork | 0.196 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a5c |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Number of reflections | 19284 | 1750 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M ammonium acetate, 0.1 M BIS-Tris pH 6.5, 25%(w/v) Polyethylene glycol 3350 |
