6A59

Structure of histone demethylase REF6 at 1.8A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-10
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9793
Spacegroup name	P 41
Unit cell lengths	63.445, 63.445, 43.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.820
R-factor	0.21562
Rwork	0.215
R-free	0.22985
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a58
RMSD bond length	0.006
RMSD bond angle	0.959
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.820	1.820
Number of reflections	15648	755
<I/σ(I)>	63.9
Completeness [%]	99.7
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG