6A4L

AcrR from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	293
Detector technology	CCD
Collection date	2015-07-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 61
Unit cell lengths	118.752, 118.752, 93.456
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.052 - 2.800
R-factor	0.215
Rwork	0.213
R-free	0.25850
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.344
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rpim	0.017	0.118
Number of reflections	18576	930
<I/σ(I)>	45.3
Completeness [%]	100.0
Redundancy	31.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1260 mM Ammonium sulfate 100 mM MES/ Sodium hydroxide pH 6.0