6A4A

Oligoribonuclease (ORN) from Colwellia psychrerythraea strain 34H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2016-06-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 41 2 2
Unit cell lengths	53.112, 53.112, 143.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.521 - 2.700
R-factor	0.2575
Rwork	0.255
R-free	0.29740
RMSD bond length	0.010
RMSD bond angle	1.129
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.110	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	6155
<I/σ(I)>	56.6
Completeness [%]	99.8
Redundancy	22.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% (w/v) PEG 3350, 0.1 M magnesium formate