6A3W
Complex structure of 4-1BB and utomilumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.727, 184.604, 90.205 |
| Unit cell angles | 90.00, 104.04, 90.00 |
Refinement procedure
| Resolution | 39.537 - 2.000 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5L36 and 5GRJ |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.596 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.537 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.066 | |
| Number of reflections | 102833 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 95.0 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M lithium chloride, 0.1M Tris, pH 8.0, 20% w/v PEG 8000 |
