6A20
Crystal Structure of auto-inhibited Kinesin-3 KIF13B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 32 |
| Unit cell lengths | 75.070, 75.070, 91.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.639 - 2.400 |
| R-factor | 0.1962 |
| Rwork | 0.193 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zbs |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.602 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.097 | 0.615 |
| Number of reflections | 22223 | 3271 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 289 | 0.2M proline, 0.1M HEPES, pH 7.5, 14% (w/v) PEG 3350 |
