6A0Z
Crystal structure of broadly neutralizing antibody 13D4 bound to H5N1 influenza hemagglutinin, HA head region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 277.15 |
| Detector technology | CCD |
| Collection date | 2014-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.245, 72.665, 76.704 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 34.670 - 2.329 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ibx 6a0x |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.586 |
| Data reduction software | HKL-2000 (V706e) |
| Data scaling software | HKL-2000 (V706e) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.329 | 2.330 |
| Rpim | 0.052 | 0.294 |
| Number of reflections | 33998 | 1670 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289.15 | 0.1 M Bis-Tris-propane pH 6.5, 0.2 M NaAc and 18% (w/v) PEG 3350 |
