6A0R
Homoserine dehydrogenase from Thermus thermophilus HB8 unliganded form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 119.197, 119.197, 144.547 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.830 |
| R-factor | 0.14971 |
| Rwork | 0.148 |
| R-free | 0.17707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xdf |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.514 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.114 | 0.359 |
| Number of reflections | 104929 | |
| <I/σ(I)> | 48.7 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Sodium formate, CAPS pH 10.0 |
