6A0P
Crystal structure of Usutu virus envelope protein in the pre-fusion state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.044, 104.337, 115.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.992 - 2.000 |
| R-factor | 0.2159 |
| Rwork | 0.214 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hg0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.645 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.808 |
| Number of reflections | 29488 | 2838 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 5.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES, pH 7.5, 10%(w/v) Polyethylene glycol 8,000, and 8%(v/v) Ethylene glycol |
