6A0L

Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from Arthrobacter globiformis, complex with maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-05
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	47.000, 115.688, 182.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.100 - 2.100
R-factor	0.25562
Rwork	0.252
R-free	0.32341
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zxg
RMSD bond length	0.015
RMSD bond angle	1.777
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.100	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.141	0.552
Number of reflections	26693	25316
<I/σ(I)>	0.115	0.022
Completeness [%]	88.6	96.7
Redundancy	4	4.6
CC(1/2)		0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M Sodium citrte, 0.22 M Ammonium sulfate, 30% (w/v) PEG 4000