6A0K

Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from Arthrobacter globiformis, complex with panose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9800
Spacegroup name	P 1 21 1
Unit cell lengths	48.293, 180.225, 62.926
Unit cell angles	90.00, 111.60, 90.00

Refinement procedure

Resolution	90.100 - 1.940
R-factor	0.18946
Rwork	0.188
R-free	0.22297
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xzg
RMSD bond length	0.020
RMSD bond angle	1.983
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.100	1.970
High resolution limit [Å]	1.940	1.940
Rmerge	0.101	0.352
Number of reflections	72961	69198
<I/σ(I)>	0.137	0.024
Completeness [%]	99.5	93
Redundancy	3.3	3
CC(1/2)		0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M Sodium citrate, 0.22 M Ammonium sulfate, 30% (w/v) PEG 4000