5ZZW
Proteobacterial origin of protein arginine methylation and regulation of Complex I assembly by MidA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2015-12-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.950, 103.540, 192.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.540 - 2.600 |
R-factor | 0.25001 |
Rwork | 0.248 |
R-free | 0.27989 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ztz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.301 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.540 | 2.693 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.186 | |
Number of reflections | 43310 | 4454 |
<I/σ(I)> | 4.9 | |
Completeness [%] | 97.7 | |
Redundancy | 6.2 | |
CC(1/2) | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M potassium sodium tartrate tetrahydrate, 0.1M BIS-TRIS pH 6.5, 10% w/v polyethylene glycol 10000 |