5ZZA

OdinProfilin/Rabbit Actin Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	54.079, 70.669, 77.717
Unit cell angles	90.00, 101.13, 90.00

Refinement procedure

Resolution	39.779 - 1.530
R-factor	0.15182
Rwork	0.150
R-free	0.17876
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yee
RMSD bond length	0.006
RMSD bond angle	1.298
Data reduction software	ADDREF
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.800	1.560
High resolution limit [Å]	1.530	1.530
Rmerge	0.051	0.314
Rmeas	0.057	0.350
Rpim	0.024	0.152
Number of reflections	85118	1968
<I/σ(I)>	12.5	2.3
Completeness [%]	98.7	81.7
Redundancy	5.2	4.9
CC(1/2)	0.999	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	100 mM Citrate, 20% PEG6000