5ZZ9

Crystal structure of Homer2 EVH1/Drebrin PPXXF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9776
Spacegroup name	P 1
Unit cell lengths	45.337, 47.808, 50.415
Unit cell angles	70.44, 83.56, 90.39

Refinement procedure

Resolution	47.153 - 2.300
R-factor	0.1934
Rwork	0.192
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ddv
RMSD bond length	0.004
RMSD bond angle	0.782
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.064	0.035	0.474
Rmeas	0.076	0.041	0.557
Rpim	0.040	0.022	0.290
Number of reflections	17008	870	797
<I/σ(I)>	5.3
Completeness [%]	96.6	98.9	94.5
Redundancy	3.5	3.5	3.6
CC(1/2)		0.997	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	50mM Ammonium fluoride, 8% w/v Polyethylene glycol 3350