5ZZ7

Redox-sensing transcriptional repressor Rex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-19
Detector	RAYONIX MX300HE
Wavelength(s)	0.90000
Spacegroup name	P 1 21 1
Unit cell lengths	50.376, 76.835, 61.346
Unit cell angles	90.00, 90.60, 90.00

Refinement procedure

Resolution	26.077 - 2.450
R-factor	0.2168
Rwork	0.215
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.423
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge		0.412
Number of reflections	17295
<I/σ(I)>	19.998
Completeness [%]	99.4
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	ammonium chloride, PEG 3350, NADH