5ZYP

Structure of the Yeast Ctr9/Paf1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9778
Spacegroup name	P 32 2 1
Unit cell lengths	95.953, 95.953, 115.498
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.976 - 2.532
R-factor	0.1989
Rwork	0.194
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.984
Data reduction software	HKL-2000 (v714)
Data scaling software	HKL-2000 (v714)
Phasing software	PHENIX ((1.10_2155_000))
Refinement software	PHENIX ((1.10_2155_000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.620
High resolution limit [Å]	2.530	2.530
Rpim	0.022	0.395
Number of reflections	35677	2051
<I/σ(I)>	29.2
Completeness [%]	99.8
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Tris-HCl, pH8.5, 0.8M Lithium sulfate, 0.01M Nickel chloride