5ZYP
Structure of the Yeast Ctr9/Paf1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9778 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.953, 95.953, 115.498 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.976 - 2.532 |
R-factor | 0.1989 |
Rwork | 0.194 |
R-free | 0.24300 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.984 |
Data reduction software | HKL-2000 (v714) |
Data scaling software | HKL-2000 (v714) |
Phasing software | PHENIX ((1.10_2155_000)) |
Refinement software | PHENIX ((1.10_2155_000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.620 |
High resolution limit [Å] | 2.530 | 2.530 |
Rpim | 0.022 | 0.395 |
Number of reflections | 35677 | 2051 |
<I/σ(I)> | 29.2 | |
Completeness [%] | 99.8 | |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1M Tris-HCl, pH8.5, 0.8M Lithium sulfate, 0.01M Nickel chloride |