5ZYO
Crystal Structure of domain-swapped Circular-Permuted YbeA (CP74) from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-12 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.00928 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.458, 88.053, 81.184 |
Unit cell angles | 90.00, 101.47, 90.00 |
Refinement procedure
Resolution | 29.520 - 1.750 |
R-factor | 0.1706 |
Rwork | 0.169 |
R-free | 0.19466 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.381 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.12_2829) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.240 | 1.810 |
High resolution limit [Å] | 1.750 | 2.160 | 1.750 |
Rmerge | 0.033 | 0.661 | 0.439 |
Rmeas | 0.039 | 0.725 | 0.525 |
Rpim | 0.020 | 0.292 | 0.285 |
Number of reflections | 65452 | 3358 | 6405 |
<I/σ(I)> | 34.82 | 2.08 | 2.13 |
Completeness [%] | 99.5 | 95.9 | 98.1 |
Redundancy | 3.7 | 5.6 | 3.1 |
CC(1/2) | 0.810 | 0.824 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 25% PEG 8000, 0.1M PIPES |
2 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 25% PEG 8000, 0.1M PIPES |