5ZYE
Crystal Structure of Glucose Isomerase Soaked with Mn2+ and Glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-09-27 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.987 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 92.749, 98.961, 102.481 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.190 - 1.400 |
R-factor | 0.16807 |
Rwork | 0.167 |
R-free | 0.18552 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y4i |
RMSD bond length | 0.021 |
RMSD bond angle | 2.060 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.190 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.065 | 0.338 |
Rmeas | 0.086 | 0.498 |
Rpim | 0.040 | 0.230 |
Number of reflections | 86795 | 4514 |
<I/σ(I)> | 30.1 | 4.43 |
Completeness [%] | 93.7 | 98.8 |
Redundancy | 4.3 | 4.2 |
CC(1/2) | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.5 | PEG 400, MgCl2 |