5ZY9
Structural basis for a tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.212, 109.633, 133.766 |
Unit cell angles | 90.00, 99.46, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
Rwork | 0.167 |
R-free | 0.20950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p3n |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.154 | 0.586 |
Rpim | 0.063 | 0.245 |
Number of reflections | 51857 | 2618 |
<I/σ(I)> | 13.6 | 2.33 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.9 | 6.7 |
CC(1/2) | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 290 | 20% (w/v) PEG 3350, 100 mM Sodium cacodylate/Hydrochloric acid pH 6.4, 200 mM Magnesium chloride |