5ZXU
Crystal structure of CurA in complex with NADPH from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 90.137, 90.137, 105.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.050 - 2.200 |
R-factor | 0.2027 |
Rwork | 0.201 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zxn |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.050 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.073 | 1.073 |
Number of reflections | 22720 | 3257 |
<I/σ(I)> | 2.2 | 0.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.4 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | sodium chloride, MES, PEG 8000 |