5ZXG
Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from Arthrobacter globiformis, ligand-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-15 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.250, 179.798, 63.412 |
Unit cell angles | 90.00, 113.61, 90.00 |
Refinement procedure
Resolution | 89.900 - 2.400 |
R-factor | 0.19103 |
Rwork | 0.188 |
R-free | 0.24812 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j0h |
RMSD bond length | 0.015 |
RMSD bond angle | 1.855 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.900 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.048 | 0.096 |
Number of reflections | 35608 | |
<I/σ(I)> | 0.232 | 0.131 |
Completeness [%] | 92.2 | 92.1 |
Redundancy | 3.2 | 3.1 |
CC(1/2) | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1 M sodium citrate, 0.22 M Ammonium sulfate, 30% (w/v) PEG4000 |