5ZXE
Structure of a consensus sequence derived from the FGF family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 35.718, 45.910, 90.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.435 - 1.300 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fgf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.770 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.910 | 45.910 | 1.260 |
| High resolution limit [Å] | 1.240 | 6.790 | 1.240 |
| Rmerge | 0.061 | 0.028 | 0.948 |
| Rmeas | 0.068 | 0.031 | 1.072 |
| Rpim | 0.030 | 0.014 | 0.484 |
| Total number of observations | 217407 | ||
| Number of reflections | 42829 | 326 | 2048 |
| <I/σ(I)> | 12 | ||
| Completeness [%] | 99.2 | 99.2 | 98.3 |
| Redundancy | 5.1 | 4.8 | 4.4 |
| CC(1/2) | 0.999 | 0.999 | 0.697 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG 8000, Ammonium sulphate |
