5ZX2
Mycobacterium tuberculosis RNA polymerase transcription initiation complex with ECF sigma factor sigma H and 7nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 129.824, 164.027, 214.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.941 - 2.800 |
R-factor | 0.2207 |
Rwork | 0.220 |
R-free | 0.25460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tw1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.621 |
Data reduction software | HKL-2000 (v716) |
Data scaling software | HKL-2000 (v716) |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.164 | 0.053 | 1.430 |
Rmeas | 0.175 | 0.057 | 1.524 |
Rpim | 0.059 | 0.020 | 0.518 |
Total number of observations | 963049 | ||
Number of reflections | 113375 | 5941 | 5564 |
<I/σ(I)> | 6.9 | ||
Completeness [%] | 99.8 | 98.1 | 99.5 |
Redundancy | 8.5 | 7.6 | 8.4 |
CC(1/2) | 0.997 | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 2% Tacsimate pH 5.0, 0.1 M Sodium citrate pH5.6, 16 % PEG3350 |