5ZWZ
Crystal structure of Arabidopsis thaliana AGDP1 AGD34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 57.177, 57.177, 206.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.192 - 2.000 |
| R-factor | 0.2007 |
| Rwork | 0.200 |
| R-free | 0.21850 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.845 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.097 | 0.241 |
| Number of reflections | 14321 | 1369 |
| <I/σ(I)> | 56 | 17.8 |
| Completeness [%] | 99.4 | 98.6 |
| Redundancy | 15.6 | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 3.5M sodium formate, 0.1M HEPES, pH 7.5 |
