5ZWY

Ribokinase from Leishmania donovani

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 61
Unit cell lengths	100.253, 100.253, 126.771
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.610 - 1.950
R-factor	0.18438
Rwork	0.183
R-free	0.21781
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fv7
RMSD bond length	0.011
RMSD bond angle	1.340
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.610	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.101	0.604
Number of reflections	52575	27813
<I/σ(I)>	0.101
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	296	Citric acid, NaCl, Glycerol