5ZWX
Crystal structure of Raphanus sativus AGDP1 AGD12 in complex with an H3K9me2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.653, 54.981, 83.576 |
| Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
| Resolution | 41.188 - 1.900 |
| R-factor | 0.1834 |
| Rwork | 0.182 |
| R-free | 0.21130 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.082 | 0.567 |
| Number of reflections | 33643 | 3346 |
| <I/σ(I)> | 21.5 | 2.5 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 15% PEG 20000, 0.1M MES, pH 6.5 |
