5ZWS
Crystal structure of apo-acyl carrier protein from Leishmania major
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-05-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.541780 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.069, 47.442, 61.128 |
Unit cell angles | 90.00, 92.81, 90.00 |
Refinement procedure
Resolution | 37.462 - 2.000 |
R-factor | 0.2529 |
Rwork | 0.252 |
R-free | 0.27220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x3o |
RMSD bond length | 0.003 |
RMSD bond angle | 0.541 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.440 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.167 | 0.526 |
Rmeas | 0.185 | |
Rpim | 0.079 | 0.257 |
Number of reflections | 11636 | |
<I/σ(I)> | 11.2 | |
Completeness [%] | 99.0 | |
Redundancy | 5.3 | |
CC(1/2) | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 294 | 0.1M Bis-Tris pH 5.5, 0.2M sodium chloride, 25% w/v polyethelene glycol 3350 |