5ZW4
Crystal structure of tRNA bound TrmR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-09-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.982, 64.982, 126.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.010 - 1.700 |
R-factor | 0.1943 |
Rwork | 0.193 |
R-free | 0.22230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gpy |
RMSD bond length | 0.009 |
RMSD bond angle | 2.003 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.010 | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
Rmerge | 0.090 | 0.065 | |
Rmeas | 0.093 | 0.067 | |
Rpim | 0.024 | 0.016 | 0.677 |
Number of reflections | 32851 | 1870 | 1400 |
<I/σ(I)> | 10.2 | ||
Completeness [%] | 94.1 | 97.3 | 81.4 |
Redundancy | 17.5 | 17 | 9.5 |
CC(1/2) | 0.997 | 0.449 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.4 M Ammonium phosphate monobasic |