5ZVA
APOBEC3F Chimeric Catalytic Domain in Complex with DNA(dC9)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-03 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.312, 91.304, 91.354 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.680 - 2.300 |
R-factor | 0.164 |
Rwork | 0.161 |
R-free | 0.20800 |
Structure solution method | SAD |
Data reduction software | ADDREF |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.680 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.121 | 0.619 |
Number of reflections | 23245 | |
<I/σ(I)> | 14 | |
Completeness [%] | 98.5 | |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.05M sodium cacodylate, pH 6.5, and 30%(v/v) 2-methyl-2,4-pentanediol(MPD) |