5ZUM
Structure of dipeptidyl-peptidase III from Corallococcus sp. strain EGB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.141, 77.850, 228.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.900 |
R-factor | 0.17634 |
Rwork | 0.174 |
R-free | 0.22361 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.515 |
Data reduction software | MOSFLM |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 9.870 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 82472 | |
<I/σ(I)> | 17.6 | |
Completeness [%] | 99.7 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.59 | 293 | 24%(w/v) PEG 3350, 0.1M sodium cacodylate acetate trihydrate pH 4.59, 0.02M TCEP hydrochioride |