5ZUI
Crystal Structure of HSP104 from Chaetomium thermophilum
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| 格子定数 [Å] | 131.182, 131.182, 137.042 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 47.382 - 2.701 |
| R因子 | 0.214 |
| Rwork | 0.211 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ciu |
| 結合角の平均二乗偏差(RMSD) [度] | 0.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.800 |
| 分解能 [Å] (高) | 2.700 | 2.700 |
| 独立反射数 | 36348 | |
| <I/σ(I)> | 31.6 | 1.2 |
| 完全性 [%] | 99.1 | 93.1 |
| 冗長性 | 10.1 | 5.1 |
| CC(1/2) | 0.998 | 0.591 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.01 M Cobalt chloride, 0.1 M MES (pH6.5), 1.8 M Ammonium sulfate |
