5ZUG
Structure of the bacterial acetate channel SatP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9709 |
Spacegroup name | P 32 |
Unit cell lengths | 99.504, 99.504, 137.824 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.533 - 2.802 |
R-factor | 0.227 |
Rwork | 0.225 |
R-free | 0.26690 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.229 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.533 | 2.900 |
High resolution limit [Å] | 2.802 | 2.802 |
Rmerge | 0.154 | 0.605 |
Number of reflections | 37419 | |
<I/σ(I)> | 3.8 | |
Completeness [%] | 99.8 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 297 | 0.05M Glycine PH 9.8, 27% PEG 300, 0.1M NaCl |