5ZUE
GTP-bound, double-stranded, curved FtsZ protofilament structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 100.526, 100.526, 138.341 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.500 - 2.700 |
R-factor | 0.211 |
Rwork | 0.210 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.520 | 2.780 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 11803 | |
<I/σ(I)> | 44.1 | |
Completeness [%] | 99.5 | |
Redundancy | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 10mM GTP, 1M sodium citrate and 0.1M imidizole, pH 8.0. |