5ZU7
Crystal structure of MERS-CoV macro domain in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.370, 39.526, 45.515 |
| Unit cell angles | 90.00, 102.37, 90.00 |
Refinement procedure
| Resolution | 29.540 - 1.705 |
| R-factor | 0.1599 |
| Rwork | 0.157 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dus |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.540 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 39892 | |
| <I/σ(I)> | 14.941 | 2.012 |
| Completeness [%] | 96.8 | 95.5 |
| Redundancy | 2.6 | 2.4 |
| CC(1/2) | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | 1.6M Sodium citrate, 0.1M HEPES sodium pH 7.5 |
