5ZU5
Crystal structure of a full length alginate lyase with CBM domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-15 |
Detector | DECTRIS PILATUS3 R CdTe 300K-W |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.272, 78.421, 112.303 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 76839 | |
<I/σ(I)> | 22 | |
Completeness [%] | 99.8 | |
Redundancy | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | 0.2M NAAC, 0.1M TRIS, 25% PEG4000, PH 8.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 288K |