5ZU2
Effect of mutation (R554A) on FAD modification in Aspergillus oryzae RIB40formate oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 155.320, 156.935, 184.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.830 - 2.441 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.23290 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.246 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.440 | 2.450 |
| Rmerge | 0.089 | 0.425 |
| Number of reflections | 81054 | 8070 |
| <I/σ(I)> | 25.4 | 4.8 |
| Completeness [%] | 97.4 | 97.9 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 Msodium acetate buffer pH 4.6, 6%(w/v) PEG 4000 |
