5ZU0
Proteobacterial origin of protein arginine methylation and regulation of Complex I assembly by MidA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2012-05-11 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9870, 0.9794, 0.9642 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.828, 105.163, 201.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.610 - 2.760 |
R-factor | 0.20186 |
Rwork | 0.200 |
R-free | 0.23583 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.528 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.610 | 2.860 |
High resolution limit [Å] | 2.760 | 2.760 |
Number of reflections | 39759 | 3811 |
<I/σ(I)> | 12.64 | |
Completeness [%] | 98.1 | |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M potassium sodium tartrate tetrahydrate, 0.1M BIS-TRIS pH 6.5, 10% w/v polyethylene glycol 10000 |