5ZTZ
Proteobacterial origin of protein arginine methylation and regulation of Complex I assembly by MidA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2012-08-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794, 0.9796, 0.9642 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.011, 105.629, 201.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.530 - 2.800 |
| R-factor | 0.21572 |
| Rwork | 0.215 |
| R-free | 0.23515 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.176 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.530 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 37830 | 3505 |
| <I/σ(I)> | 6.98 | |
| Completeness [%] | 96.8 | |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% ethylene glycol, 0.1M HEPES pH 7.5, 10% PEG 8000 |
