5ZTM

Crystal structure of MLE dsRBDs in complex with roX2 (R2H1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	H 3 2
Unit cell lengths	108.479, 108.479, 347.061
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.548 - 2.899
R-factor	0.1935
Rwork	0.191
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vyx 3vyy
RMSD bond length	0.009
RMSD bond angle	1.183
Data reduction software	HKL-2000
Data scaling software	HKL-2000 (3.24)
Phasing software	MOLREP
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.548	3.000
High resolution limit [Å]	2.899	2.900
Rmerge	0.120	0.787
Rmeas		0.198
Rpim		0.906
Number of reflections	17911
<I/σ(I)>	2.7
Completeness [%]	99.9
Redundancy	9.4
CC(1/2)		0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200mM lithium citrate tribasic tetrahydrate, 20% PEG 3350