5ZTL
Non-cryogenic structure of light-driven chloride pump having an NTQ motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 294 |
Detector technology | PIXEL |
Collection date | 2017-12-14 |
Detector | CS-PAD CXI-2 |
Wavelength(s) | 1.3 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.360, 50.090, 69.400 |
Unit cell angles | 90.00, 109.65, 90.00 |
Refinement procedure
Resolution | 18.520 - 1.850 |
R-factor | 0.242 |
Rwork | 0.240 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g28 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.520 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 28973 | |
<I/σ(I)> | 4 | |
Completeness [%] | 100.0 | |
Redundancy | 52.6 | |
CC(1/2) | 0.965 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6 | 294 | 30% PEG 500 DME, 0.1M sodium chloride, 0.1M MES (pH 6.0) |