5ZTK
Synchrotron structure of light-driven chloride pump having an NTQ motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-06-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.756, 49.402, 69.327 |
| Unit cell angles | 90.00, 109.85, 90.00 |
Refinement procedure
| Resolution | 23.630 - 1.750 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g28 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.630 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 32667 | |
| <I/σ(I)> | 24.47 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6 | 294 | 30% PEG 500 DME, 0.1M sodium chloride, 0.1M MES (pH 6.0) |
