5ZTJ

Crystal Structure of GyraseA C-Terminal Domain from Salmonella typhi at 2.4A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	273
Detector technology	CCD
Collection date	2017-08-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9919
Spacegroup name	P 41 21 2
Unit cell lengths	79.150, 79.150, 87.357
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.125 - 2.400
R-factor	0.1927
Rwork	0.190
R-free	0.24180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zi0
RMSD bond length	0.003
RMSD bond angle	0.639
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.660	2.640
High resolution limit [Å]	2.400	2.400
Number of reflections	20743	3331
<I/σ(I)>	14.89	2.87
Completeness [%]	99.9	99.5
Redundancy	5.6
CC(1/2)	0.998	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Magnesium Chloride hexahydrate, glycerol