5ZTH

Crystal structure of spRlmCD with U1939loop RNA at 3.24 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	47.947, 95.531, 114.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.240
R-factor	0.2159
Rwork	0.213
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xj1
RMSD bond length	0.006
RMSD bond angle	1.004
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.300
High resolution limit [Å]	3.240	8.780	3.240
Rmerge	0.314	0.098	0.863
Rmeas	0.332	0.105	0.913
Rpim	0.106	0.035	0.294
Number of reflections	8963	521	427
<I/σ(I)>	1.8
Completeness [%]	100.0	100	100
Redundancy	9.6	8.1	9.5
CC(1/2)		0.995	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Sodium dihydrogen Phosphate 20% (w/v) PEG3350